Journal: Molecular Systems Biology

Article Title: Molecular profiling reveals features of clinical immunity and immunosuppression in asymptomatic P. falciparum malaria

doi: 10.15252/msb.202110824

Figure Lengend Snippet:

Article Snippet: 176Yb‐conjugated mouse anti‐human CD127 , Fluidigm , Clone A019D5; Cat# 3176004B.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Marker, Infection, Purification, Flow Cytometry, Blocking Assay, Generated, Recombinant, Saline, Staining, Software, Cytometry

Journal: Scientific Reports

Article Title: IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study

doi: 10.1038/s41598-022-13063-x

Figure Lengend Snippet: Systemic CD3 + CD127 + lymphocytes in CAP and asymptomatic adults (ASN) ( A ), according to age, sex ( B ) and outcome ( C ). Data are shown as median and 95% CI. P values were calculated by Mann–Whitney test.

Article Snippet: For the surface staining of peripheral blood mononuclear cells (1 × 10 6 ), the followings antibodies of BD Pharmigen™ were used: APC mouse anti-human CD3 (555342), PerCPCy5.5 mouse anti-human CD4 (341654), FITC mouse anti-human CD8 (555634) and BV421 mouse anti-human CD127 (562436).

Techniques: MANN-WHITNEY

Journal: Scientific Reports

Article Title: IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study

doi: 10.1038/s41598-022-13063-x

Figure Lengend Snippet: CD3 + CD127 + lymphocytes in blood from adults with CAP according to SNP. Data are shown as median and 95% CI. p > 0.1, by t test.

Article Snippet: For the surface staining of peripheral blood mononuclear cells (1 × 10 6 ), the followings antibodies of BD Pharmigen™ were used: APC mouse anti-human CD3 (555342), PerCPCy5.5 mouse anti-human CD4 (341654), FITC mouse anti-human CD8 (555634) and BV421 mouse anti-human CD127 (562436).

Techniques:

Journal: Scientific Reports

Article Title: IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study

doi: 10.1038/s41598-022-13063-x

Figure Lengend Snippet: Systemic CD4 + CD127 + ( A ) and CD8 + CD127 + ( B ) T lymphocytes in CAP adult according to severity by PSI. Data are shown as median and 95% CI. P values were obtained by t test.

Article Snippet: For the surface staining of peripheral blood mononuclear cells (1 × 10 6 ), the followings antibodies of BD Pharmigen™ were used: APC mouse anti-human CD3 (555342), PerCPCy5.5 mouse anti-human CD4 (341654), FITC mouse anti-human CD8 (555634) and BV421 mouse anti-human CD127 (562436).

Techniques:

Journal: Scientific Reports

Article Title: IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study

doi: 10.1038/s41598-022-13063-x

Figure Lengend Snippet: Plasmatic soluble IL7R from asymptomatic and CAP adults ( A ), according to age, sex ( B ) and outcome ( C ). Data are shown as median and 95% CI. P values were calculated by Mann–Whitney test.

Article Snippet: For the surface staining of peripheral blood mononuclear cells (1 × 10 6 ), the followings antibodies of BD Pharmigen™ were used: APC mouse anti-human CD3 (555342), PerCPCy5.5 mouse anti-human CD4 (341654), FITC mouse anti-human CD8 (555634) and BV421 mouse anti-human CD127 (562436).

Techniques: MANN-WHITNEY

Journal: Scientific Reports

Article Title: IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study

doi: 10.1038/s41598-022-13063-x

Figure Lengend Snippet: Soluble IL7R according to the genotypes of the three SNPs in adult CAP. Median, 25–75% percentile, minimum and maximum are indicated. P values were obtained by Mann–Whitney test, (*) p < 0.05, (**) p < 0.01, (***) p < 0.001, (****) p < 0.0001.

Article Snippet: For the surface staining of peripheral blood mononuclear cells (1 × 10 6 ), the followings antibodies of BD Pharmigen™ were used: APC mouse anti-human CD3 (555342), PerCPCy5.5 mouse anti-human CD4 (341654), FITC mouse anti-human CD8 (555634) and BV421 mouse anti-human CD127 (562436).

Techniques: MANN-WHITNEY

Journal: BMC cancer

Article Title: Molecular subtyping based on immune cell marker genes predicts prognosis and therapeutic response in patients with lung adenocarcinoma.

doi: 10.1186/s12885-023-11579-7

Figure Lengend Snippet: Fig. 7 LIDscore model genes analysis. (A) Expressions of LGR4, GOLM1, CYP24A1, SFTPB, COL1A1, HLA-DQA1, MS4A7, PPARG, and IL7R in different cell types as classified by the t-SNE algorithm. (B) Model genes mRNA expression in 59 paired sample tissues in TCGA. (C) Model gene protein expression levels based on immunohistochemical staining. (D) Kaplan–Meier curves demonstrate survival differences for high- and low-expression level subgroups based on the model gene

Article Snippet: Methods are as follows: xylene was used to deparaffinize sections, which were then immersed in EDTA antigen extraction buffer for antigen retrieval, blocked with 3% hydrogen peroxide, incubated with rabbit polyclonal antibody to IL7R (1:100; biorbyt; P16871) overnight, followed by secondary antibodies incubation and staining with DAB.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: The Journal of experimental medicine

Article Title: Single-cell profiling reveals unique features of diabetogenic T cells in anti-PD-1-induced type 1 diabetes mice.

doi: 10.1084/jem.20221920

Figure Lengend Snippet: Figure 3. CD4+ T cells in the pancreas show increased proliferation in anti-PD-1-induced T1D. (A) UMAP visualization of integrated CD4+ T cells detected in the pancreas. Points represent individual cells and colors denote cluster classification as labeled. (B) UMAP visualization of integrated CD4+ T cells detected in the pancreas of each treatment group. Dots represent individual cells with the color representing the cluster classification and size corresponding to the size of the clonotype. Colors denote functional annotations that correspond to the same labels shown in A. (C) Stacked bar plots denoting the percentage of cells from each treatment group within individual clusters (left panel). Heatmap showing the number of cells detected in each cluster and treatment group (middle panel) and −log10 adjusted P values from a Fisher’s exact test (right panel). (D) Violin plots quantifying percent TIGIT+ staining within CD4+FoxP3+ Treg in the pancreas measured by flow cytometry. IgG-Spt, P = 0.0235. (E) Violin plots showing gMFI of T-bet in CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas by flow cytometry analysis. IgG-PD1, P = 0.0008; Spt-PD1, P = 0.0020. (F) Violin plots quantifying percent CD127+ staining within CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas measured by flow cytometry. IgG-Spt, P < 0.0001; Spt-PD1, P = 0.0012. (G) Violin plots showing percent BCL-2+ in CD4+FoxP3−CD11ahiCD62L−

Article Snippet: Cells were preincubated with TruStain Fc Receptor Block (anti-mouse CD16/ CD32, clone 93; BioLegend) and then labeled with extracellular antibodies from BioLegend: CD8α (53-6.7), PD-1 (RMP1-30), CD29 (HMβ 1-1; BioLegend), CD48 (HM48-1), CD94 (18d3), NKG2D (CX5), CD39 (Duha59), NKG2A (16A11), TIM-3 (RMT323), SLAMF7 (4G2), TIGIT (IG9), CX3CR1 (SA011F11), CD62L (MEL-14), CD44 (IM7), CD127 (A7R34), CD49D (R1-2), CD38 (90), CXCR3 (CXCR3-173); BD Biosciences: TCR-β (H57-597), CD4 (GK1.5), CD11a (M17/4), CD8b (H35-17.2); and Bio-Rad: LAG-3 (C9B7W).

Techniques: Labeling, Functional Assay, Staining, Flow Cytometry

Journal: eLife

Article Title: Mesenchymal stem cell suppresses the efficacy of CAR-T toward killing lymphoma cells by modulating the microenvironment through stanniocalcin-1

doi: 10.7554/eLife.82934

Figure Lengend Snippet: The cell co-culture contained chimeric antigen receptor modified T cell (CAR-T) cells, Pfeiffer cells, M2 macrophages, and control or stanniocalcin-1 (STC1) knockdown hMSCs in a ratio of 1:3:1:1. After 24 hr (or 48 hr for cytotoxicity) incubation, the following analysis was conducted: ( A ) The impact of hMSC (w/o STC1) on the cytotoxicity of CAR-T toward Pfeiffer cells; ( B ) FACS analysis of CD4 + and CD8 + composition. ( C ) Quantitation of the FACS data on CD4 + and CD8 + ; ( D ) FACS analysis of Treg + cells (CD4 + CD127 + CD25 + ); ( E ) Quantitation of Treg + cells. ( F ) Western blot analysis of indoleamine 2,3-dioxygenase (IDO) and programmed cell death-ligand 1 (PD-L1) expression in the cell co-culture. Data in bar graphs are presented as the mean ± SD from three independent experiments (p values are as indicated, n=3). Figure 2—source data 1. in Excel file. Figure 2—source data 2. in Excel file. Figure 2—source data 3. in Excel file. Figure 2—source data 4. Labeled original blots of . Figure 2—source data 5. Unlabeled original blots of .

Article Snippet: The expression of CD4, CD8, CD127, and CD25 in CAR-T cells was analyzed using flow cytometry with the following fluorochrome-conjugated monoclonal/polyclonal antibodies (all from Caprico Biotechnologies): anti-human CD4 (CD004210403), anti-human CD8 (CD008210301), anti-human CD127 (CD127210501), and anti-human CD25 (CD025210301).

Techniques: Co-Culture Assay, Modification, Control, Knockdown, Incubation, Quantitation Assay, Western Blot, Expressing, Labeling

Journal: Frontiers in Immunology

Article Title: Grass pollen allergoids conjugated with mannan for subcutaneous and sublingual immunotherapy: a dose-finding study

doi: 10.3389/fimmu.2024.1431351

Figure Lengend Snippet: Variations (fold-change) in circulating Treg cells and grass allergen-specific IL-10-producing cells after four months of treatment depending on dose and route of administration (subcutaneous: left panels; sublingual: right panels) (A) Percentage of Treg cells (CD4 + CD127 - CD25 + FOXP3 + ) relative to total CD4 + T cells. (B) Number of Phleum-specific spot-forming cells (SFC) secreting IL-10 after their expansion in vitro . (C) Data as in panel B but regrouping of subjects as indicated. Results are expressed as fold-change (median; interquartile range) relative to baseline. Comparison with placebo, Unpaired T test or Mann-Whitney test: * p<0.05.

Article Snippet: For Treg cell analysis, PBMC were first subjected to surface staining with anti-human CD4-PerCP, CD127-PE, and CD25-APC (Miltenyi Biotec).

Techniques: In Vitro, Comparison, MANN-WHITNEY